mouse anti tsg101 Search Results


mouse anti-tsg101  (Becton Dickinson)
90
Becton Dickinson mouse anti-tsg101
A. Removal of uromodulin after 2000 x g centrifugation. Urine samples (30 μl in sample buffer 4x) before (start urine) and after 2000 x g centrifugation (2000g, sup.) were loaded onto the gel. The pellet obtained after 2000 x g centrifugation was solubilized in a similar volume of water as the volume of the supernatant, and 30 μl in sample buffer 4x were loaded onto the gel (2000g, pellet). Asterisks in Coomassie stained gels (upper part) indicate uromodulin as confirmed by Western blot (lower part). Sup: Supernatant. Std: molecular weight (kD) standard. B. Urinary exosomes were isolated by ultracentrifugation and 10 μg were run in a 4-20% SDS-PAGE. Exosomal proteins were Coomassie stained. Std: molecular weight (kD) standard. C. Urinary exosomes labeled with mouse anti-CD63 followed by rabbit-anti-mouse, and then by 10 nm Protein A-gold conjugates were inspected by electron microscopy. D. Identification of CD9, CD81 and <t>Tsg101</t> in urinary exosomes (2 μg) by Western blot. E . Five exosome markers detected by MS, quantified by Top 3 TIC ( n = 15; ± SEM).
Mouse Anti Tsg101, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse anti-tsg101 - by Bioz Stars, 2026-02
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mouse anti-tsg101  (HansaBioMed ltd)
90
HansaBioMed ltd mouse anti-tsg101
A. Removal of uromodulin after 2000 x g centrifugation. Urine samples (30 μl in sample buffer 4x) before (start urine) and after 2000 x g centrifugation (2000g, sup.) were loaded onto the gel. The pellet obtained after 2000 x g centrifugation was solubilized in a similar volume of water as the volume of the supernatant, and 30 μl in sample buffer 4x were loaded onto the gel (2000g, pellet). Asterisks in Coomassie stained gels (upper part) indicate uromodulin as confirmed by Western blot (lower part). Sup: Supernatant. Std: molecular weight (kD) standard. B. Urinary exosomes were isolated by ultracentrifugation and 10 μg were run in a 4-20% SDS-PAGE. Exosomal proteins were Coomassie stained. Std: molecular weight (kD) standard. C. Urinary exosomes labeled with mouse anti-CD63 followed by rabbit-anti-mouse, and then by 10 nm Protein A-gold conjugates were inspected by electron microscopy. D. Identification of CD9, CD81 and <t>Tsg101</t> in urinary exosomes (2 μg) by Western blot. E . Five exosome markers detected by MS, quantified by Top 3 TIC ( n = 15; ± SEM).
Mouse Anti Tsg101, supplied by HansaBioMed ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti-tsg101/product/HansaBioMed ltd
Average 90 stars, based on 1 article reviews
mouse anti-tsg101 - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier

Image Search Results


A. Removal of uromodulin after 2000 x g centrifugation. Urine samples (30 μl in sample buffer 4x) before (start urine) and after 2000 x g centrifugation (2000g, sup.) were loaded onto the gel. The pellet obtained after 2000 x g centrifugation was solubilized in a similar volume of water as the volume of the supernatant, and 30 μl in sample buffer 4x were loaded onto the gel (2000g, pellet). Asterisks in Coomassie stained gels (upper part) indicate uromodulin as confirmed by Western blot (lower part). Sup: Supernatant. Std: molecular weight (kD) standard. B. Urinary exosomes were isolated by ultracentrifugation and 10 μg were run in a 4-20% SDS-PAGE. Exosomal proteins were Coomassie stained. Std: molecular weight (kD) standard. C. Urinary exosomes labeled with mouse anti-CD63 followed by rabbit-anti-mouse, and then by 10 nm Protein A-gold conjugates were inspected by electron microscopy. D. Identification of CD9, CD81 and Tsg101 in urinary exosomes (2 μg) by Western blot. E . Five exosome markers detected by MS, quantified by Top 3 TIC ( n = 15; ± SEM).

Journal: Oncotarget

Article Title: Identification of prostate cancer biomarkers in urinary exosomes

doi:

Figure Lengend Snippet: A. Removal of uromodulin after 2000 x g centrifugation. Urine samples (30 μl in sample buffer 4x) before (start urine) and after 2000 x g centrifugation (2000g, sup.) were loaded onto the gel. The pellet obtained after 2000 x g centrifugation was solubilized in a similar volume of water as the volume of the supernatant, and 30 μl in sample buffer 4x were loaded onto the gel (2000g, pellet). Asterisks in Coomassie stained gels (upper part) indicate uromodulin as confirmed by Western blot (lower part). Sup: Supernatant. Std: molecular weight (kD) standard. B. Urinary exosomes were isolated by ultracentrifugation and 10 μg were run in a 4-20% SDS-PAGE. Exosomal proteins were Coomassie stained. Std: molecular weight (kD) standard. C. Urinary exosomes labeled with mouse anti-CD63 followed by rabbit-anti-mouse, and then by 10 nm Protein A-gold conjugates were inspected by electron microscopy. D. Identification of CD9, CD81 and Tsg101 in urinary exosomes (2 μg) by Western blot. E . Five exosome markers detected by MS, quantified by Top 3 TIC ( n = 15; ± SEM).

Article Snippet: The antibodies used for Western blotting were: mouse anti-Tsg101 (BD Biosciences, Heidelberg, Germany); rabbit anti-CD9 (Abcam, Cambridge, UK); mouse anti-CD81 (Ancell Corporation, Bayport, MN, USA), rabbit anti-uromodulin (St. Cruz Biotechnology Inc., Dallas, TX, USA).

Techniques: Centrifugation, Staining, Western Blot, Molecular Weight, Isolation, SDS Page, Labeling, Electron Microscopy